The South participants in the 2010 Macaulay Cup have been confirmed by the Macaulay Association.
Scottish Hydro Premier Division sides Kyles Athletic, Oban Camanachd, Inveraray, Bute, and Glenorchy, join Glasgow Mid Argyll, Kilmory, and Oban Celtic from the Marine Harvest South Division 1. Lochside Rovers are unable to enter as they are Oban Camanachd’s second team.
The North participants are Kingussie, Newtonmore, Fort William, Glenurquhart and Lochaber from the Scottish Hydro Premier Division and Lovat, Kilmallie and Skye Camanachd from Marine Harvest North Division 1.